Highly purified Candida utilis plasma membranes prepared by phase partition
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چکیده
منابع مشابه
Isolation of purified plasma membranes from cultured cells and hepatomas by two-phase partition and preparative free-flow electrophoresis.
This report describes and documents the isolation of plasma membranes from hepatomas and tissue culture cells by aqueous two-phase partition. The method used previously for normal liver was effective, rapid, and reproducible. Preparations from both cells and hepatomas were more than 90% plasma membrane-derived based on electron microscope morphometry and assays of marker enzyme activities. Rela...
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Highly purified mitochondrial ribosomes (mitoribosomes) have been obtained from the yeast Candida utilis. Sedimentation analysis in sucrose gradients made in 5 mM MgCl(2), 1 mM Tris, pH 7.4 and 50 mM KCl clearly distinguishes mitoribosomes (72S) from cytoplasmic ribosomes (cytoribosomes) (78S). Mitoribosomes are completely dissociated into 50S and 36S subunits at 10(-4)M MgCl(2) whereas complet...
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1. Plasma membranes were isolated from crude nuclear sediments from mouse and rat liver by a rate-dependent centrifugation through a sucrose density gradient contained in the ;A' type zonal rotor. 2. The membranes were further purified by isopycnic centrifugation, and characterized enzymically, chemically and morphologically. 3. When the plasma-membrane fraction of sucrose density 1.17g/cm(3) w...
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Starved cells of Candida utilis accumulated Zn2+ by two different processes. The first was a rapid, energy- and temperature-independent system that probably represented binding to the cell surface. The cells also possessed an energy-, pH-, and temperature-dependent system that was capable of accumulating much greater quantities of the cation than the binding process. The energy-dependent system...
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Under steady-state conditions, the maximum rate of lactic acid removal was found to be 588 mg/h per liter of continuous culture.
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1983
ISSN: 0378-1097
DOI: 10.1016/0378-1097(83)90124-6